Niagara Falls and Niagara Falls, New York are linked to major highways in Canada and the United States respectively, with the Queen Elizabeth Way acting as a major artery between Toronto and Fort Erie, Ontario. Highway 420 (along with Niagara Regional Road 420) connect the Rainbow Bridge to the QEW. The Whirlpool Bridge is located at the end of Bridge Street. The Niagara Parkway is a road operated under the Niagara Parks Commission which connects Niagara-on-the-Lake to Fort Erie via Niagara Falls.
Niagara Falls formerly had King's Highways passing through the city. These included:
- The original routing of Highway 3, (which later became Highway 3A,) which ended at the Whirlpool Rapids Bridge via River Road
- Highway 8, which ended at the Whirlpool Rapids Bridge via Bridge Street
- Highway 20, which ended at the Honeymoon Bridge and later the Rainbow Bridge via Lundy's Lane and Clifton Hill
- The Queen Elizabeth Way followed Roberts Street and Newman Hill to the Rainbow Bridge—later renamed Highway 420
- Buffalo Niagara International Airport in Cheektowaga, New York.
- Toronto Pearson International Airport in Mississauga, Ontario - the primary international airport
- Hamilton/John C. Munro International Airport in Mount Hope, Ontario.
- Niagara Falls International Airport in Niagara Falls, New York
- St. Catharines/Niagara District Airport in Niagara-on-the-Lake, Ontario
Via Rail Canada and Amtrak jointly provide one train per day in each direction. This is The Maple Leaf which operates between Toronto Union, Niagara, Buffalo, Albany and New York's Penn station), arriving and departing out of the Niagara Falls station. In the summer it offers a bike train service on a limited schedule.
In summer 2009, Go Transit Started a pilot project providing weekend and holiday train service from Toronto to Niagara falls From Mid June to mid October. These GO Trains run seasonally between Toronto Union Station and Niagara Falls at weekends.
At other times, regular hourly GO train services are provided between Toronto Union and Burlington station, where connecting bus services operate to and from the rail station at Niagara.
- Coach Canada has daily runs to and from Toronto and Buffalo, New York.
- GO Transit offers daily bus service between Niagara and Burlington GO Station.
- Greyhound Canada has daily runs to and from Toronto and Buffalo, New York.
- Megabus has daily runs on its route to New York City starting in Toronto.
- Niagara Transit is the public transit operator in the city.
Cabs and shuttle buses
- Buffalo Airport Shuttle is a reservation based shuttle that operates from the Buffalo Airport to and from Niagara Falls, Buffalo, Hamilton, and Toronto.
- Niagara Livery Service is a taxi/limo company in Niagara.
- 5-0 is a local cab service. A taxi shuttle provides transfers to airports from Buffalo, New York to Niagara Falls, Ontario and Toronto, Ontario.
- Niagara Falls Taxi is a local taxi service from Buffalo, New York and Toronto, Ontario airports back to Niagara.
- Elite Taxi is a local taxi service that provides regular and wheelchair accessible taxi service to and from Niagara Falls, ON. Specialists in airport transfers (Buffalo, Hamilton, Toronto, Niagara Falls, NY).
The City of Niagara Falls is working toward Bike Friendly designation and providing more resources to encourage active transportation.